Systems biotechnology for protein production in Pichia pastoris
نویسندگان
چکیده
منابع مشابه
STRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris
Introduction. Yeasts are widely used in production of recombinant proteins (r-proteins) of medical or industrial interest. For the production of a biomolecule in a host a creative metabolic engineering design is achieved, and then optimized both on the molecular genetic and fermentation level, by taking into account the properties of the product, the host organism and the expression cassette. A...
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Background: The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which i...
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The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins. Recent developments with respect to the Pichia expression system have had an impact on not only the expression levels that can be achieved, but also the bioactivity of various heterologous proteins. We review here some of these recent developments, as well as strateg...
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Conventionally, two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate γ-glutamylcysteine.
متن کاملImprovement in the downstream processing of rhGH production in Pichia pastoris
Production of human proteins in Pichia pastoris has significant advantages. However, there is still need for improvement in various stages of its downstream processing like clarification and purification. In fact downstream processes are usually the most critical part of production of biotech products. This work aimed to evaluate the effect of two steps added to the downstream processes ...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2017
ISSN: 1567-1364
DOI: 10.1093/femsyr/fox068